cell lines/media

Note: Media and cell lines are not "for sale", and we are unable to offer these to investigators outside of the University of Michigan community. Dollar amounts listed below reflect partial costs that we recover using a shortcode-to-shortcode transfer.  

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LWRN-conditioned Media*

$30/50mLs (prices include only costs for time/supplies to generate the conditioned media)

APPLICATION NOTES:

1. Generated from ATCC cell line CRL-3276 according to Miyoshi et al. (Nat. Protocols, 2013)

2. This media is not 'ready to use' (Complete Media).  See the protocols page for generating Complete Media.

3. Quality Control: TopFlash luciferase reporter assay to determine WNT signaling activity, Mycoplasma testing. Details available upon request. 

TO ORDER: FILL IN FORM BELOW.  Payment will be made via short-code-to-shortcode transfer on a quarterly basis. Please indicate how many 50mL aliquots you would like, and shortcode to be charged.


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Human intestinal epithelium as 3D organoids

Ready to use for experiments from one of our "standard" well characterized lines. 

$10/well - 24 well plate sized well

TO ORDER: FILL IN FORM BELOW.  Payment will be made via short-code-to-shortcode transfer on a quarterly basis. Please indicate how many wells you would like, and shortcode to be charged.


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Primary human tissue lines.       

Want to grow organoids in your own lab? We have a bank of well characterized lines (click here).

Prior to transfer of organoids, a Simple letter agreement (SLA) will be signed by TTML and receiving investigator.

TO ORDER: FILL IN FORM BELOW.  Payment will be made via short-code-to-shortcode transfer. Please indicate shortcode to be charged. At this time we can accept only auxiliary account shortcodes (begin with "2......."). Other in-kind reimbursement arrangements can be made if you do not have an auxiliary account. We also hope to soon have in place a process to receive NIH and other shortcodes.

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Complete Gastrointestinal Growth Media*

$50/50mLs (prices include only costs for time/supplies to generate the complete media)

APPLICATION NOTES:

1. Complete Media is 'ready to use'. Made with LWRN-conditioned Media plus a host of other supplements/factors. Full formulation can be found here. 

2. This Complete Media has been used to successfully grow epithelium-only stomach, small intestine and colonic organoids from adult human tissue.

3. Quality Control: TopFlash luciferase reporter assay to determine WNT signaling activity, Mycoplasma testing. Details available upon request. 

*Comparing Laboratory Prepared Medium and a Commercial Medium Alternative for Expansion of Human Colonoid Cultures

TO ORDER: FILL IN FORM BELOW.  Payment will be made via short-code-to-shortcode transfer on a quarterly basis. Please indicate how many 50mL aliquots you would like, and shortcode to be charged.


Adult_enteroid_3D-and-2D.jpg

Human intestinal epithelium as 2D monolayer

Ready to use for experiments from one of our "standard" well characterized lines. 

$15/well - 48 well plate sized well

TO ORDER: FILL IN FORM BELOW.  Payment will be made via short-code-to-shortcode transfer on a quarterly basis. Please indicate how many wells you would like, and shortcode to be charged.


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